Journal: Cells

Article Title: Combined TGF-β3 and FGF-2 Stimulation Enhances Chondrogenic Potential of Ovine Bone Marrow-Derived MSCs

doi: 10.3390/cells14131013

Figure Lengend Snippet: Alcian blue staining of ovine BM-MSCs in 2D culture in ( a ) chondrogenic differentiation medium ( b ) with FGF-2, ( c ) TGF-β3, ( d ) FGF-2, and TGF-β3. ( e ) Effect of TGF-β3 and FGF-2 cytokines on the intensity of Alcian blue staining in 2D culture for 21 days of culture. Three independent experiments in triplicates were performed. * p < 0.05. Ovine BM-MSCs treated with FGF-2 and TGF-β3 exhibit the highest chondrogenic differentiation potential.

Article Snippet: After an overnight attachment, the medium was replaced with the chondrogenic differentiation medium (PromoCell, Heidelberg, Germany, cat. no. C-28016) either supplemented or not with FGF-2 or TGF-β3 or both.

Techniques: Staining

Journal: Cells

Article Title: Combined TGF-β3 and FGF-2 Stimulation Enhances Chondrogenic Potential of Ovine Bone Marrow-Derived MSCs

doi: 10.3390/cells14131013

Figure Lengend Snippet: Alcian blue staining of ovine (BM-MSCs) in 3D culture in ( a ) chondrogenic differentiation medium ( b ) supplemented with FGF-2 ( c ) TGF-β3, ( d ) FGF-2, and TGF-β3. ( e ) Intensity of Alcian blue staining of sheep BM-MSCs in 3D culture for 21 days. Three experiments in triplicates were performed. * p ˂ 0.05, ** p ˂ 0.005, and *** p ˂ 0.0001. Treatment of sheep BM-MSCs with both FGF-2 and TGF-β3 enhances 3D chondrogenic differentiation.

Article Snippet: After an overnight attachment, the medium was replaced with the chondrogenic differentiation medium (PromoCell, Heidelberg, Germany, cat. no. C-28016) either supplemented or not with FGF-2 or TGF-β3 or both.

Techniques: Staining

Journal: Cells

Article Title: Combined TGF-β3 and FGF-2 Stimulation Enhances Chondrogenic Potential of Ovine Bone Marrow-Derived MSCs

doi: 10.3390/cells14131013

Figure Lengend Snippet: Real-time PCR analysis for markers of chondrogenic differentiation of sheep BM-MSCs treated with TGF-β3 and/or FGF-2 for 7, 14, and 21 days. Chad , Comp , and Sox 5 ( a – c ) characterize the early stage of chondrogenesis; gene expression of Agg , Col IX , Sox 9 , and Fmod ( d – g ) characterize the late stage of chondrogenic differentiation. * p ˂ 0.05, ** p ˂ 0.005, and *** p ˂ 0.0001. Three experiments with two biological replicates were conducted. Treatment of ovine BM-MSCs with the cytokines FGF-2 and TGF-β3 increase the expression of both early and late chondrogenic marker genes.

Article Snippet: After an overnight attachment, the medium was replaced with the chondrogenic differentiation medium (PromoCell, Heidelberg, Germany, cat. no. C-28016) either supplemented or not with FGF-2 or TGF-β3 or both.

Techniques: Real-time Polymerase Chain Reaction, Gene Expression, Expressing, Marker

Journal: PLoS ONE

Article Title: Lung Fibroblasts Share Mesenchymal Stem Cell Features Which Are Altered in Chronic Obstructive Pulmonary Disease via the Overactivation of the Hedgehog Signaling Pathway

doi: 10.1371/journal.pone.0121579

Figure Lengend Snippet: (a)Rate of lung fibroblast conversion into myofibroblasts after 3, 4 and 5 days exposure to TGF-β1. * p<0.05 compared to untreated cells. # p<0.05 compared to non- smoker (C-NS) and smoker controls (C-S) exposed to TGF-β1 for 4 or 5 days. (b) Oil-red O staining of, MSC, DF and lung fibroblasts from C-NS, C-S and COPD subjects12 days after exposure to adipogenic differentiation medium. (c)Alcian blue staining of MSC, DF and lung fibroblasts at day 24 following chondrogenesis initiation. (d,e) (D, E) Comparative real-time PCR analysis of mRNA expression of genes encoding for (d) adipogenic peroxysome proliferative activated receptor gamma 2 (PPAR-γ 2; early), lipoprotein lipase (LPL; late) and (e)chondrogenic Sox-9 marker. (f) Alizarin red staining of cells following 17 days of osteoblastic induction and (g)real-time PCR analysis of mRNA expression of osteoblastic alkaline phosphatase (ALP, early) and osteocalcin (OC, late) markers. (d,e,g) Results represent means ± SEM of at least 3 independent experiments performed with donor fibroblasts. NS: non significant; * p<0.05, ** p<0.01. MSC, mesenchymal stem cells; DF, dermal fibroblast; C-NS, non-smokers controls; C-S, smoker controls; COPD, smokers with COPD.

Article Snippet: Chondrogenic differentiation was carried out using Mesenchymal Stem Cell Chondrogenic Differentiation Medium (PromoCell, Heidelberg, Germany).

Techniques: Staining, Real-time Polymerase Chain Reaction, Expressing, Marker